Oxidative phosphorylation in cardiolipin-lacking yeast mitochondria

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Oxidative phosphorylation in cardiolipin-lacking yeast mitochondria.

The role of cardiolipin in mitochondrial energy transformation was studied by comparing oxidative phosphorylation in the Saccharomyces cerevisiae cardiolipin synthase null mutant crd1Delta, and in isogenic wild type. Oxygen consumption experiments and membrane potential kinetics during the phosphorylation cycle in isolated mitochondria indicated that the absence of cardiolipin causes only a mod...

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Oxidative phosphorylation with endogenous substrates of mitochondria.

During recent studies of oxidative phosphorylation in rat liver mitochondria (2, 3)) we observed that preparations isolated with meticulous care catalyzed a significant uptake of inorganic orthophosphate in the absence of any added substrate. Although incidental observations of endogenous activity of isolated mitochondria have been noted by others (P9), no extensive study of this phenomenon has...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 2000

ISSN: 0264-6021

DOI: 10.1042/0264-6021:3470687